PCNA antibody (Internal Region)

Catalog No. ABIN2470261

This Rabbit Polyclonal antibody specifically detects PCNA in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse, Rat, Chicken, Monkey, Dog, Cow, Xenopus laevis and Fish.

Quick Overview for PCNA antibody (Internal Region) (ABIN2470261)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Cow, Xenopus laevis, Fish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-PCNA Antibody

Binding Specificity: Internal Region

Purification: Immunoaffinity Chromatography

Immunogen: PCNA antibody was raised against an internal region of PCNA (Human).

Application Details

Application Notes: PCNA antibody can be used in ELISA starting at 1:000 - 1:1000, and immunohistochemistry starting at 10 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies avoid freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store PCNA antibody at 4 °C or -20 °C.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the processibility of DNA polymerase during elongation of the leading strand. PCNA is expressed in the nucleus of all proliferating cells and is pivotal for DNA synthesis and cell cycle progression. In response to DNA damage, PCNA is mono-ubiquitinated and is involved in mismatch-provoked excision. PCNA is a useful marker for DNA synthesis and is highly conserved among most species.

Gene ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy