COL6A1 antibody

Catalog No. ABIN2470329

Product Details anti-COL6A1 Antibody

Target: COL6A1 (Collagen, Type VI, alpha 1 (COL6A1))

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL6A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Purification: Immunoaffinity Chromatography

Immunogen: COL6A1 antibody was raised against Collagen Type VI from human and bovine placenta (Human).

Application Details

Application Notes: COL6A1 antibody can be used in immunohistochemistry starting at 1.25 - 2.5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies avoid freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store COL6A1 antibody at 4 °C or -20 °C.

Target Details for COL6A1

Target: COL6A1 (Collagen, Type VI, alpha 1 (COL6A1))

Alternative Name: COL6A1 (COL6A1 Products)

Synonyms: CBR antibody, SDR21C1 antibody, hCBR1 antibody, OPLL antibody, AI747156 antibody, Col6a-1 antibody, RGD1565398 antibody, COL6A1 antibody, carbonyl reductase 1 antibody, collagen type VI alpha 1 chain antibody, collagen, type VI, alpha 1 antibody, collagen, type VI, alpha 1 L homeolog antibody, collagen type VI alpha 3 chain antibody, collagen alpha-1(VI) chain antibody, CBR1 antibody, COL6A1 antibody, Col6a1 antibody, col6a1.L antibody, COL6A3 antibody, col6a1 antibody, LOC100623720 antibody

Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.

Gene ID: 1291

UniProt: P12109

Pathways: Growth Factor Binding, SARS-CoV-2 Protein Interactome