This antibody is specific for Rat Collagen type II (100 %). Cross Reactions: Rat Collagen I, III and V: < 0.1 % Rat Elastin, Rat-Fibronectin: < 0.1 % (solid phase RIA at 1/100 dilution).
Purification
Ion Exchange Chromatography
Immunogen
Purified collagen type II from fetal rat cartilage
COL2A1
Reactivity: Human
IHC, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
IF: 1: 40. Immunohistochemistry on frozen and paraffin sections: (P) 1: 100 - 1: 500, 60 min at RT or2-8C over night. ELISA. RIA. Positive control: rat cartilage. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 0.5 mL distilled water. For further dilution use appropriate antibody diluent.
Concentration
>/= 1.0 mg/mL
Handling Advice
Avoid repeated freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.
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Background
Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type II Collagen is an alpha1(II)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and various tumours.Synonyms: Alpha-1 type II collagen, COL2A1