BRAF antibody (pThr753)

Catalog No. ABIN2567054

This Rabbit Polyclonal antibody specifically detects BRAF in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BRAF antibody (pThr753) (ABIN2567054)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BRAF Antibody

Binding Specificity: pThr753

Specificity: The antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 753

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: B-Raf (phospho Thr753) antibody was raised against a peptide sequence around phosphorylation site of threonine 753 (P-K-T (p)-P-L) derived from Human B-Raf.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: B-Raf

Background: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Molecular Weight: 84 kDa

Gene ID: 673

NCBI Accession: NP_004324

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy