FOXO1/3/4-pan antibody (pThr24, pThr32)

Catalog No. ABIN2568146

Product Details anti-FOXO1/3/4-pan Antibody

Target: FOXO1/3/4-pan (FOXO1/3/4) (Forkhead Box O1/3/4 (FOXO1/3/4))

Binding Specificity: pThr24, pThr32

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1/3/4-pan antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody detects endogenous levels of FOXO1/3/4-pan only when phosphorylated at threonine 24/32.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: FOXO1/3/4-pan (phospho Thr24/32) antibody was raised against a peptide sequence around phosphorylation site of threonine 24/32 (S-C-T (p)-W-P) derived from Human FOXO1/3/4-pan .

Isotype: IgG

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for FOXO1/3/4-pan

Target: FOXO1/3/4-pan (FOXO1/3/4) (Forkhead Box O1/3/4 (FOXO1/3/4))

Alternative Name: FOXO1/3/4-Pan (FOXO1/3/4 Products)

Background: Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts syngernistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells.

Molecular Weight: 78 kDa

Gene ID: 2308, 4303

NCBI Accession: NP_002006

UniProt: Q12778, O43524, P98177