CD74 antibody (Internal Region)
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- Target See all CD74 Antibodies
- CD74 (HLA-DR-gamma (CD74))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This CD74 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Purpose
- CD74
- Sequence
- HSLEQKPTDA PPK
- Specificity
- This antibody is expected to recognize isoform a (NP_001020330.1) and isoform b (NP_004346.1).
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Isotype
- IgG
- Top Product
- Discover our top product CD74 Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5 μg/mL.
Western Blot: Approx 33 kDa band observed in Human Tonsil lysates (calculated MW of 33.5 kDa according to NP_001020330.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB: Meza-Romero
Peptide ELISA: antibody detection limit dilution 1:128000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- CD74 (HLA-DR-gamma (CD74))
- Alternative Name
- CD74 (CD74 Products)
- Synonyms
- DHLAG antibody, HLADG antibody, II antibody, Ia-GAMMA antibody, CLIP antibody, Ii antibody, INVG34 antibody, Iclp-1 antibody, iclp1 antibody, wu:fb11f09 antibody, wu:fb12a01 antibody, wu:fb13d06 antibody, wu:fk94e07 antibody, Iclp-2 antibody, cd74 antibody, iclp2 antibody, zgc:123161 antibody, CD74 molecule antibody, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody, CD74 molecule, major histocompatibility complex, class II invariant chain a antibody, CD74 molecule, major histocompatibility complex, class II invariant chain b antibody, CD74 antibody, Cd74 antibody, cd74a antibody, cd74b antibody
- Background
- CD74, DHLAG, HLADG, Ia-GAMMA, CD74 Molecule, major histocompatibility complex, class II invariant chain, CD74 antigen, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), HLA-DR-gamma, Ia-associated invar
- Gene ID
- 972
- NCBI Accession
- NP_001020330, NP_004346
- Pathways
- Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints
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