VCP antibody (Internal Region)

Catalog No. ABIN2613406

Product Details anti-VCP Antibody

Target: VCP (Valosin Containing Protein (VCP))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VCP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: CDC48 / YDL126C

Sequence: HTKNMKLADD VDLE

Specificity: This antibody may cross react in many other species.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat, Saccharomyces cerevisiae

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 5 μg/mL.

Western Blot: Approx 100 kDa band observed in lysates of cell line HeLa (calculated MW of 92.0 kDa according to NP_010157.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VCP

Target: VCP (Valosin Containing Protein (VCP))

Alternative Name: CDC48 (VCP Products)

Synonyms: ALS14 antibody, IBMPFD antibody, TERA antibody, p97 antibody, CDC48 antibody, wu:fd16d05 antibody, wu:fj63d11 antibody, p93 antibody, 3110001E05 antibody, p97/VCP antibody, 22.26 antibody, 7.12 antibody, Apple antibody, BcDNA.GM02885 antibody, BcDNA:GM02885 antibody, CG2331 antibody, DmTER94 antibody, Dmel\CG2331 antibody, I antibody, Ter94 antibody, Tera antibody, VCP antibody, VCP ATPase antibody, VCP/p97 antibody, Vcp antibody, anon-WO2004063362.65 antibody, anon-WO2004063362.67 antibody, cdc48 antibody, dTERA antibody, dVCP antibody, dmTERA antibody, dvcp antibody, l(2)03775 antibody, l(2)46CFf antibody, l(2)46CFs antibody, l(2)46Ch antibody, pTER94 antibody, ter94 antibody, vcp antibody, FANCG antibody, valosin containing protein antibody, valosin containing protein S homeolog antibody, valosin-containing protein antibody, CG2331 gene product from transcript CG2331-RA antibody, zgc:136908 antibody, transitional endoplasmic reticulum ATPase antibody, VCP antibody, vcp.S antibody, vcp antibody, Vcp antibody, Bm1_56585 antibody, LOC100136246 antibody, TER94 antibody, zgc:136908 antibody, LOC101109680 antibody

Background: CDC48, CDC48p, YDL126C, VCP, transitional endoplasmic reticulum ATPase, valosin containing protein, p97, TERA

Gene ID: 7415, 269523, 116643

NCBI Accession: NP_010157

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway