PPP2R1A antibody (N-Term)

Catalog No. ABIN264939

Product Details anti-PPP2R1A Antibody

Target: PPP2R1A (Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A))

Binding Specificity: AA 7-19, N-Term

Reactivity: Human, Mouse, Rat, Cow

Host: Sheep

Clonality: Polyclonal

Conjugate: This PPP2R1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Sequence: NH2-Asp-Asp-Ser-Leu-Try-Pro-Ile-Ala-Val-Leu-Ile--Asp-Glu-COOH

Specificity: This antibody recognizes Serine/Threonine Protein Phosphatase 2A/A.

Purification: Purified

Immunogen: A peptide conjugated to KLH corresponding to amino acids 7-19 sequence (N-terminal) of PP2A/A regulatory subunit having a MW of 65kD

Isotype: IgG

Application Details

Application Notes: Western Blot: 1-5 μg. Immunoprecipitation: often used in combination with Ig purified anti pp2A/C toimmunoprecipitate heterotrimeric pp2A. Immunocytochemistry: 1/500-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4 and 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PPP2R1A

Target: PPP2R1A (Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A))

Alternative Name: PPP2R1A (PPP2R1A Products)

Synonyms: PP2A-Aalpha antibody, PP2AAALPHA antibody, PR65A antibody, PR65 antibody, pp2a-aalpha antibody, pp2aaalpha antibody, ppp2r1a antibody, pr65a antibody, wu:fa02h04 antibody, zgc:56296 antibody, 6330556D22Rik antibody, PP2A antibody, ppp2r1a-a antibody, ppp2r1a-b antibody, protein phosphatase 2 scaffold subunit Aalpha antibody, protein phosphatase 2 scaffold subunit A alpha antibody, protein phosphatase 2 regulatory subunit A, alpha L homeolog antibody, protein phosphatase 2 regulatory subunit A, alpha antibody, protein phosphatase 2, regulatory subunit A, beta a antibody, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform antibody, protein phosphatase 2, regulatory subunit A, alpha isoform antibody, protein phosphatase 2, regulatory subunit A, alpha antibody, protein phosphatase 2 regulatory subunit A, alpha S homeolog antibody, PPP2R1A antibody, Ppp2r1a antibody, ppp2r1a.L antibody, ppp2r1a antibody, ppp2r1ba antibody, LOC583227 antibody, ppp2r1a.S antibody

Background: Protein phosphatase 2A (PP2A) is the most abundant phosphatase specific for serine and threonine phosphorylation in mammalian cells. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit alpha (PPP2R1A) serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory beta subunit.Synonyms: PP2A subunit A PR65-alpha isoform, PP2A subunit A R1-alpha isoform, Protein phosphatase 2A, Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

Gene ID: 5518

NCBI Accession: NP_055040

UniProt: P30153

Pathways: PI3K-Akt Signaling, Mitotic G1-G1/S Phases, M Phase, Hepatitis C, Toll-Like Receptors Cascades