MMP14 antibody

Catalog No. ABIN265525

Product Details anti-MMP14 Antibody

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of MMP-14 protein. (region surrounding Pro509)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for MMP14

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Alternative Name: MMP-14 (MMP14 Products)

Synonyms: MMP1 antibody, MMP-14 antibody, MMP-X1 antibody, MT-MMP antibody, MT-MMP 1 antibody, MT1-MMP antibody, MT1MMP antibody, MTMMP1 antibody, mmp-x1 antibody, mt1-mmp antibody, mtmmp1 antibody, AI325305 antibody, MT-MMP-1 antibody, sabe antibody, Mt1-mmp antibody, MT-MMPbeta antibody, cb386 antibody, sb:cb386 antibody, wu:fb72b09 antibody, mmp14 antibody, matrix metallopeptidase 14 antibody, matrix metallopeptidase 14 (membrane-inserted) antibody, matrix metallopeptidase 14b (membrane-inserted) antibody, matrix metallopeptidase 14a (membrane-inserted) antibody, matrix metallopeptidase 14 L homeolog antibody, MMP14 antibody, mmp14 antibody, Mmp14 antibody, mmp14b antibody, mmp14a antibody, mmp14.L antibody

Background: The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. Membrane-type matrix metalloproteinases, including MT-MMP-1 (also designated MMP-14), MT-MMP-2 (also designated MMP-15), MT-MMP-3 (also designated MMP-16) and MT-MMP-4 (also designated MMP-17) are type I membrane proteins that function to activate other MMPs. MT-MMP activation appears to be mediated by members of the proprotein convertase family, suggesting that a proprotein convertase/MT-MMP/MMP cascade may be involved in the regulation of ECM turnover.Synonyms: MMP-X1, MMP14, Matrix metalloproteinase-14, Membrane-type-1 matrix metalloproteinase

Molecular Weight: approx. 53, 65 kDa

Gene ID: 4323

NCBI Accession: NP_004986

UniProt: P50281

Pathways: Autophagy