Rpb1 CTD antibody (pSer5)

Catalog No. ABIN2668520

Cited in 3+ publications. The Rabbit Polyclonal anti-Rpb1 CTD antibody (ABIN2668520) specifically detects Rpb1 CTD in WB, ChIP and ChIP-seq. The antibody is reactive with Human and Mouse samples.

Quick Overview for Rpb1 CTD antibody (pSer5) (ABIN2668520)

Target: Rpb1 CTD (RNA Polymerase II CTD)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rpb1 CTD antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Rpb1 CTD Antibody

Binding Specificity: pSer5

Purpose: RNA pol II CTD phospho Ser5 antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This RNA pol II CTD phospho Ser5 antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at serine 5.

Isotype: IgG

Application Details

Application Notes: ChIP: 4 - 10 μg per ChIP ChIP-Seq: 4 µL each WB: 0.5 - 2 μg/mL dilution For RNA pol II CTD phospho Ser5, we also offer AbFlex RNA pol II CTD phospho Ser5 Recombinant Antibody (rAb). For details, see Catalog No. 91119.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Rpb1 CTD antibody (ABIN2668520)

Young, Hillyer, Hokamp, Fitzpatrick, Konstantinov, Welty, Ness, Werner-Washburne, Fleming, Osley: "Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast." in: BMC genomics, Vol. 18, Issue 1, pp. 107, (2017) (PubMed).

Ke, McKnight, Gracey Maniar, Sun, Callaway, Majnik, Lane, Cohen: "IUGR increases chromatin-remodeling factor Brg1 expression and binding to GR exon 1.7 promoter in newborn male rat hippocampus." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 309, Issue 2, pp. R119-27, (2015) (PubMed).

Lee, Chen, Huang, Stargell: "The head module of Mediator directs activation of preloaded RNAPII in vivo." in: Nucleic acids research, Vol. 41, Issue 22, pp. 10124-34, (2013) (PubMed).

Target Details for Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Alternative Name: RNA Pol II CTD

Background: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at Serine 5 and Serine 2. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.

Molecular Weight: 210 kDa

Gene ID: 5430

NCBI Accession: NP_000928