Rpb1 CTD antibody (pSer5)

Catalog No. ABIN4889651

This Rabbit Polyclonal antibody specifically detects Rpb1 CTD in WB, IF, ICC and ChIP. It exhibits reactivity toward Human and Mouse. It has been mentioned in 5+ publications

Quick Overview for Rpb1 CTD antibody (pSer5) (ABIN4889651)

Target: Rpb1 CTD (RNA Polymerase II CTD)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rpb1 CTD antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Product Details anti-Rpb1 CTD Antibody

Binding Specificity: pSer5

Purpose: RNA pol II CTD phospho Ser5 antibody (pAb)

Purification: Unpurified

Immunogen: This RNA pol II CTD phospho Ser5 antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at serine 5.

Application Details

Application Notes: ChIP: 10 µL per ChIP ICC/IF: 1:250 - 1:500 dilution WB: 1:500 - 1:2,000 dilution For RNA pol II CTD phospho Ser5, we also offer AbFlex RNA pol II CTD phospho Ser5 Recombinant Antibody (rAb). For details, see Catalog No. 91119.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Rpb1 CTD antibody (ABIN4889651)

Schubert, Weisshart: "Abundance and distribution of RNA polymerase II in Arabidopsis interphase nuclei." in: Journal of experimental botany, Vol. 66, Issue 6, pp. 1687-98, (2015) (PubMed).

Albini, Coutinho, Malecova, Giordani, Savchenko, Forcales, Puri: "Epigenetic reprogramming of human embryonic stem cells into skeletal muscle cells and generation of contractile myospheres." in: Cell reports, Vol. 3, Issue 3, pp. 661-70, (2013) (PubMed).

Sanei, Pickering, Kumke, Nasuda, Houben: "Loss of centromeric histone H3 (CENH3) from centromeres precedes uniparental chromosome elimination in interspecific barley hybrids." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 33, pp. E498-505, (2011) (PubMed).

Nelissen, De Groeve, Fleury, Neyt, Bruno, Bitonti, Vandenbussche, Van der Straeten, Yamaguchi, Tsukaya, Witters, De Jaeger, Houben, Van Lijsebettens: "Plant Elongator regulates auxin-related genes during RNA polymerase II transcription elongation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 4, pp. 1678-83, (2010) (PubMed).

Blythe, Cha, Tadjuidje, Heasman, Klein: "beta-Catenin primes organizer gene expression by recruiting a histone H3 arginine 8 methyltransferase, Prmt2." in: Developmental cell, Vol. 19, Issue 2, pp. 220-31, (2010) (PubMed).

Target Details for Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Alternative Name: RNA Pol II CTD

Background: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at Serine 5 and Serine 2. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.

Molecular Weight: 210 kDa

Gene ID: 5430

NCBI Accession: NP_000928