CD28 antibody

Catalog No. ABIN2689148

Product Details anti-CD28 Antibody

Target: CD28

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: The CD28.2 monoclonal antibody specifically binds to CD28, a 44 kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells. CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions. It has been suggested that CD28 initiates and regulates a separate and distinct signal transduction pathway from those stimulated by the TCR complex. Additionally, it has been reported that CD28 antibody clones vary in their ability to stimulate T cells to produce IL-2 and increase intracellular Ca2+ concentration. This finding suggests the existence of functionally distinct subregions on the CD28 Molecule. CD28.2 has been demonstrated to bind to the same molecule as clone L293, another CD28 mAb, and has been reported to induce Ca2+ influx in Jurkat T cells. Profile of peripheral blood lymphocytes analyzed by flow cytometry. Second step staining using FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988).

BD Pharmingen™ Purified Mouse Anti-Human CD28 - Purified - Clone CD28.2 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CD28 Transfected Cell Line

Clone: CD28-2

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD28 antibody (ABIN2689148)

Verwilghen, Vandenberghe, Wallays, de Boer, Anthony, Panayi, Ceuppens: "Simultaneous ligation of CD5 and CD28 on resting T lymphocytes induces T cell activation in the absence of T cell receptor/CD3 occupancy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 3, pp. 835-46, (1993) (PubMed).

Target Details for CD28

Target: CD28

Alternative Name: CD28 (CD28 Products)

Synonyms: Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody

Background: Synonyms: CD28 antigen, T44, Tp44, TP44

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response