IL12 antibody

Catalog No. ABIN2689729

Product Details anti-IL12 Antibody

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL12 antibody is un-conjugated

Application: Blocking Antibody (Inhibition)

Brand: BD Pharmingen™

Characteristics: The C11.5 antibody reacts with human IL-12 p40 monomer and p70 heterodimer, but not p35 monomer. The immunogen used to generate the C11.5 hybridoma was the CHO-expressed recombinant human IL-12 p70 heterodimer. p40 has also been described as a subunit of IL-23 and thus it is possible that the C11.5 antibody will crossreact with IL-23. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of IL-12 p40/p70 by activated CD14+ human PBMCs. Ficoll-separated human PBMCs were primed for 2 hrs with recombinant human IFN-γ (10 ng/mL, Cat. No. 554616), then activated with IFN-γ (10 ng/mL) and LPS (100 ng/mL, Sigma) in the presence of 2 μM GolgiStop™ (Cat. No. 554724) for an additional 22 hr. Cells were harvested, stained with FITC-mouse anti human CD14 antibody (Cat. No. 555397), fixed, permeabilized, and then stained with 0.125 μg of PE-C11.5 antibody (Cat. No. 554575), following Pharmingen's staining protocol (left panel). The data reflect gating on monocytes, based on forward and side scattered light signals. To demonstrate specificity of staining, the binding of the PE-C11.5 antibody was blocked by preincubation of the fixed/permeabilized cells with the unlabeled C11.5 antibody (5 μg, Cat. No. 554573) prior to staining (right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls.

BD Pharmingen™ Purified Mouse Anti-Human IL-12 (p40/p70) - Purified - Clone C11.5 - Isotype Mouse IgG1 - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: CHO-expressed recombinant human IL-12 p70 heterodimer

Clone: C11-5

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL12 antibody (ABIN2689729)

Oppmann, Lesley, Blom, Timans, Xu, Hunte, Vega, Yu, Wang, Singh, Zonin, Vaisberg, Churakova, Liu, Gorman, Wagner, Zurawski, Liu, Abrams, Moore, Rennick, de Waal-Malefyt, Hannum, Bazan, Kastelein: "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12." in: Immunity, Vol. 13, Issue 5, pp. 715-25, (2001) (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

DAndrea, Rengaraju, Valiante, Chehimi, Kubin, Aste, Chan, Kobayashi, Young, Nickbarg: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, (1992) (PubMed).

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12 p40,p70 (IL12 Products)

Synonyms: CLMF antibody, CLMF2 antibody, IL-12B antibody, NKSF antibody, NKSF2 antibody, p40 antibody, Il-12b antibody, Il12p40 antibody, Il-12p40 antibody, Il12 antibody, IL-12p40 antibody, IL-12 antibody, LOC100217488 antibody, IL-12p35 antibody, Il-12a antibody, Ll12a antibody, p35 antibody, IL12p40 antibody, IL12B antibody, IL-12 p35 antibody, interleukin 12B antibody, interleukin 12A antibody, interleukin 12b antibody, interleukin 12a antibody, Interleukin 12 level antibody, IL12B antibody, IL12A antibody, Il12b antibody, Il12a antibody, IL12 antibody, il12a antibody

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome