IL12 antibody (Biotin)

Catalog No. ABIN2689732

Product Details anti-IL12 Antibody (Biotin)

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL12 antibody is conjugated to Biotin

Application: ELISA

Brand: BD Pharmingen™

Characteristics: The C8.6 antibody reacts with human IL-12 p40 monomers, homodimers, and p70 heterodimers, but not p35 subunits. The immunogen used to generate the C8.6 hybridoma was the purified p40 subunit of human IL-12. The C8.6 antibody is a neutralizing antibody and has been reported to strongly inhibit three different biological activities of IL-12 (i.e., IFN-γ induction), mitogenic effects on PHA blasts, and enhancement of NK cell-mediated cytotoxicity. C8.6 has been shown to immunoprecipitate IL-23. This antibody is routinely tested by ELISA as a detection antibody. Other applications were tested during antibody development only or reported in the literature. Human IL-12 p70 Protein Quantitation by ELISA. Rat anti-human IL-12 p70 specific antibody 20C2 (Cat. No. 555065) was paired with a biotinylated anti-IL-12 p40/p70 detection (Cat. No. 554660) to measure specifically human IL-12 p70 protein, using recombinant human IL-12 p70 (Cat. No. 554613), as the ELISA standard.

BD Pharmingen™ Biotin Mouse Anti-Human IL-12 (p40/p70) - Biotin - Clone C8.6 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified p40 Subunit of Human IL-12

Clone: C8-6

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IL12 antibody (Biotin) (ABIN2689732)

Oppmann, Lesley, Blom, Timans, Xu, Hunte, Vega, Yu, Wang, Singh, Zonin, Vaisberg, Churakova, Liu, Gorman, Wagner, Zurawski, Liu, Abrams, Moore, Rennick, de Waal-Malefyt, Hannum, Bazan, Kastelein: "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12." in: Immunity, Vol. 13, Issue 5, pp. 715-25, (2001) (PubMed).

DAndrea, Rengaraju, Valiante, Chehimi, Kubin, Aste, Chan, Kobayashi, Young, Nickbarg: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, (1992) (PubMed).

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12 p40,p70 (IL12 Products)

Synonyms: CLMF antibody, CLMF2 antibody, IL-12B antibody, NKSF antibody, NKSF2 antibody, p40 antibody, Il-12b antibody, Il12p40 antibody, Il-12p40 antibody, Il12 antibody, IL-12p40 antibody, IL-12 antibody, LOC100217488 antibody, IL-12p35 antibody, Il-12a antibody, Ll12a antibody, p35 antibody, IL12p40 antibody, IL12B antibody, IL-12 p35 antibody, interleukin 12B antibody, interleukin 12A antibody, interleukin 12b antibody, interleukin 12a antibody, Interleukin 12 level antibody, IL12B antibody, IL12A antibody, Il12b antibody, Il12a antibody, IL12 antibody, il12a antibody

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome