MDM2 antibody (pSer166)

Catalog No. ABIN7299346

This Rabbit Polyclonal antibody specifically detects MDM2 in WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MDM2 antibody (pSer166) (ABIN7299346)

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MDM2 Antibody

Binding Specificity: pSer166

Purpose: Rabbit polyclonal antibody to MDM2 (Phospho-S166)

Specificity: Recognizes endogenous levels of MDM2 protein only when phosphorylated at S166.

Characteristics: Rabbit polyclonal antibody to MDM2 (pS166)

Purification: The antibody was purified by immunogen affinity chromatography.

Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S166 of human MDM2 protein. The exact sequence is proprietary.

Application Details

Application Notes: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Alternative Name: MDM2

Background: E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2

Gene ID: 4193

UniProt: Q00987

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway