CD45RA antibody

Catalog No. ABIN7304834

This Mouse Monoclonal antibody specifically detects CD45RA in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for CD45RA antibody (ABIN7304834)

Target: CD45RA

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RA antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HI100

Product Details anti-CD45RA Antibody

Purpose: Mouse monoclonal antibody to CD45RA

Specificity: Recognizes human CD45RA

Characteristics: Mouse monoclonal antibody to CD45RA

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD45RA.

Isotype: IgG2b

Application Details

Application Notes: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CD45RA

Target: CD45RA

Alternative Name: CD45RA

Background: CD45, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD antigen CD45

Gene ID: 5788

UniProt: P08575