PTPN1 antibody

Catalog No. ABIN272074

Product Details anti-PTPN1 Antibody

Target: PTPN1 (Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTPN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of PTP1B protein. (region surrounding Tyr46)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for PTPN1

Target: PTPN1 (Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1))

Alternative Name: PTPN1 (PTPN1 Products)

Synonyms: PTPN1 antibody, PTP1B antibody, PTP-1B antibody, PTP-HA2 antibody, Ptp antibody, ptp1b antibody, wu:fk54h03 antibody, CPTP1 antibody, protein tyrosine phosphatase, non-receptor type 1 antibody, protein tyrosine phosphatase, non-receptor type 1 L homeolog antibody, PTPN1 antibody, Ptpn1 antibody, ptpn1 antibody, ptpn1.L antibody

Background: The phosphorylation of proteins at tyrosine residues has long been recognized as an important regulatory component of signal transduction. This is a reversible process, involving both enzymes that phosphorylate proteins on tyrosine residues as well as a rapidly expanding family of protein tyrosinephosphatases. These latter enzymes bear little resemblance to either theprotein serine and protein threonine phosphatases or to the acid and alkalinephosphatases. In most tissues, the major PTPase is a vanadate- and molybdate-sensitive protein of approximately 40 kDa molecular weight. On the basis of sequence analysis, PTP1B expressed in human placenta exhibits similarities both with the common leukocyte antigen (CD45) and with LAR, ahomolog of the neural adhesion molecule (NCAM). PTPase 1B is synthesized as a 435 amino acid precursor protein which is cleaved to generate the active 321 amino acid enzyme.Synonyms: PTP-1B, PTP1B, Protein Tyrosin Phosphatase, Protein-tyrosine phosphatase 1B, Tyrosine-protein phosphatase non-receptor type 1

Molecular Weight: approx. 49 kDa

Gene ID: 5770

NCBI Accession: NP_002818

UniProt: P18031

Pathways: TLR Signaling, Response to Growth Hormone Stimulus, ER-Nucleus Signaling, Platelet-derived growth Factor Receptor Signaling