PFKL antibody
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- Target See all PFKL Antibodies
- PFKL (Phosphofructokinase, Liver (PFKL))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PFKL antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of PFK-B protein. (region surrounding Glu724)
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- Affinity Chromatography using epitope-specific immunogen.
- Top Product
- Discover our top product PFKL Primary Antibody
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- Application Notes
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- PFKL (Phosphofructokinase, Liver (PFKL))
- Alternative Name
- PFKL (PFKL Products)
- Synonyms
- PFK-B antibody, AA407869 antibody, phosphofructokinase, liver type antibody, phosphofructokinase, liver, B-type antibody, PFKL antibody, Pfkl antibody
- Background
- Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).Synonyms: 6-phosphofructokinase liver, PFK-B, Phosphofructokinase 1, Phosphohexokinase
- Molecular Weight
- approx. 85 kDa
- Gene ID
- 5211
- NCBI Accession
- NP_002617
- UniProt
- P17858
- Pathways
- Negative Regulation of Hormone Secretion, Warburg Effect
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