XRCC6/XRCC5 antibody

Catalog No. ABIN2742517

Product Details anti-XRCC6/XRCC5 Antibody

Target: XRCC6/XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5/6 (XRCC6/XRCC5))

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This XRCC6/XRCC5 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificity: Human Ku70+Ku80 / XRCC6+XRCC5

No Cross-Reactivity: Chicken, Mouse (Murine), Rat (Rattus)

Purification: Protein G purified

Immunogen: Nuclear extract of human HL-60 cells.

Type of Immunogen: Cells

Clone: KU729

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo (0.5 - 1 μg/10 x10⁶), ICC (0.25 - 0.5 μg/mL), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP

Usage: This antibody recognizes a dimer of two proteins of 70  kDa (Ku70) and ~80  kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460  kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. Immunocytochemistry (Acetone-fixed cells) : 0.25-0.5 μg/mL for 30 minutes at RT.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for XRCC6/XRCC5

Target: XRCC6/XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5/6 (XRCC6/XRCC5))

Alternative Name: Ku70+Ku80 / XRCC6+XRCC5 (XRCC6/XRCC5 Products)

Background: This antibody detects: Ku70+Ku80 / XRCC6+XRCC5