XRCC6/XRCC5 antibody

Catalog No. ABIN6938789

Product Details anti-XRCC6/XRCC5 Antibody

Target: XRCC6/XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5/6 (XRCC6/XRCC5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This XRCC6/XRCC5 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificity: Recognizes a dimer of two proteins of 70 kDa and ~80 kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

No Cross-Reactivity: Chicken, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Human. Primates. Does not react with mouse, rat and chicken.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Nuclear extract of human HL-60 cells

Clone: KU729

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunocytochemistry (Acetone-fixed cells) (0.5-1.0 μg/mL for 30 minutes at RT),Optimal dilution for a specific application should be determined.

Positive_Control: K562, HL-60, HEp-2 or HeLa cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for XRCC6/XRCC5

Target: XRCC6/XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5/6 (XRCC6/XRCC5))

Alternative Name: XRCC6 (p70) & XRCC5 (p80) (XRCC6/XRCC5 Products)

Background: 70 kDa / 80 kDa subunit of Ku antigen, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, G22P1, Lupus Ku autoantigen protein p70, ML8, Thyroid autoantigen 70kD (Ku antigen), Thyroid-lupus autoantigen (TLAA), X-ray repair cross-complementing protein 6 (XRCC6), ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, KARP1, KUB2, Lupus Ku autoantigen protein p86, Nuclear factor IV (NFIV), X-ray repair cross-complementing protein 5 (XRCC5),Ku (p70/p80) (Nuclear Marker)
Cellular localisation: Nuclear

Molecular Weight: 70kDa & 80kDa