NONO antibody (AA 200-250) (FITC)

Catalog No. ABIN2746949

Product Details anti-NONO Antibody (FITC)

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Binding Specificity: AA 200-250

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NONO antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): The antibody does not cross react to any other cellular protein.

Characteristics: NonO-selective antibodies were generated against a 17mer peptide taken form the mid- region of the NonO protein. The NonO-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The anti-NonO-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The Supplier provides NonO Western blot positive control samples in SDS-PAGE sample buffer.
Synonyms: Non-POU domain containing Octamer-binding protein, NonO

Purification: Affinity Purified

Immunogen: Synthetic peptide taken within amino acid region 200-250 on human NonO protein.

Isotype: IgG

Application Details

Application Notes: Antibodies were tested in ELISA and western blotting applications at 1:500 dilution using ABIN1686765 samples. Antibody dilutions for these antibodies are for reference only, investigators are expected to determine the optimal conditions. Application of this antibody in other protocols has not yet tested.
WB: > 1:500
IMM & IP pull-down assays: N.D. .
IHC: N.D. .
Investigators using this antibody in protocols other than listed above can request a complimentary sample of this antibody. N.D. not necessarily means the antibody is not suitable for that application, it simply means we have not yet characterized the antibody for that application.
The antibody labels a strong band of NonO at 57 kDa in ABIN1686765 samples.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55-0.75 μg/μL

Storage: -20 °C

Storage Comment: Storage of very dilute antibody solutions is not recommended.

Target Details for NONO

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Alternative Name: Nmt55 / p54nrb (NONO Products)

Synonyms: NMT55 antibody, NRB54 antibody, P54 antibody, P54NRB antibody, nmt55 antibody, nrb54 antibody, p54nrb antibody, xp54nrb antibody, AA407051 antibody, AV149256 antibody, nonA antibody, non-POU domain containing octamer binding antibody, non-POU domain containing, octamer-binding antibody, non-POU domain containing, octamer binding L homeolog antibody, non-POU-domain-containing, octamer binding protein antibody, NONO antibody, Nono antibody, nono.L antibody

Background: NonO is a 57 kDa ubiquitously expressed nucleic acid binding protein with their main function in transcriptional and post transcriptional gene regulatory functions as well as DNA repair. It binds to both DNA and RNA via its functionally separable domains. It has a tandem arrayed RNP and a putative HTH followed by a region rich in both acidic and basic amino acid residues. NonO binds to double-stranded DNA by its HTH domain and it binds with less sequence specificity by its RNP domain to RNA (or single-stranded DNA). Binding of RNA and dsDNA can occur simultaneously indicating that the RNP and HTH domains function independently. NonO belongs to the Drosophila/human splicing (DBHS) family that includes SFPQ, PSPC1 and invertebrate NONA and Hrp65. All DBHS proteins have a conserved region of approximately 300 AA. They differ only in the N- and the C-terminal extensions differing in their length with its extra polypeptide consisting of polyproline/glutamine-rich low-complexity regions and functional sequences including nuclear localization signals. NonO proteins are predominantly nuclear and they stimulate no homologous end joining (NHEJ) and represses homologous recombination. Defects in the NonO protein have significantly increased the chromosomal aberrations in human cells indicating it importance in DNA repair as its major function. They are involved in the regulation of circadian rhythm, carcinogenesis, and progression of cancer. NonO protein also interacts with RASD1 and regulates the circadian rhythms in biological clocks.

UniProt: Q15233