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HLA-DR antibody (PE)

Name: HLA-DR antibody (PE)
Brand: antibodies-online
SKU: ABIN2749150
Price: 584.13 USD
Availability: InStock

This anti-HLA-DR antibody is a Mouse Monoclonal antibody detecting HLA-DR in FACS. Suitable for Human, Dog and Non-Human Primate. This Primary Antibody has been cited in 7+ publications.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR (L243) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (HLA-DR antibody (PE))

FACS

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD3 (UCHT1) APC antibody (10 μL reagent / 100 μL of peripheral whole blood) and anti-human HLA-DR (L243) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (HLA-DR antibody (PE))

FACS

Separation of human CD3 negative HLA-DR positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-DR (L243) PE antibody (10 μL reagent / 100 μL of peripheral whole blood). (HLA-DR antibody (PE))

FACS

3 images

(7 references)

Catalog No. ABIN2749150

Quick Overview for HLA-DR antibody (PE) (ABIN2749150)

Target

HLA-DR

Reactivity

Human, Dog, Non-Human Primate

Host

Mouse

Clonality

Monoclonal

Conjugate

This HLA-DR antibody is conjugated to PE

Application

Flow Cytometry (FACS)

Clone

L243

Purpose

Anti-HLA-DR PE

Specificity

The mouse monoclonal antibody L243 recognizes specifically an extracellular epitope on HLA-DR molecules, both peptide-loaded and empty.

Cross-Reactivity (Details)

Human, Non-Human Primates, Canine (Dog)

Purification

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen

Human B lymphocytes

Isotype

IgG2a kappa
anti-HLA-DR (HLA-DR) antibody (FITC)
HLA-DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-12 FITC

anti-HLA-DR (HLA-DR) antibody
HLA-DR Reactivity: Human FACS, WB, IHC, ISt, StM Host: Mouse Monoclonal TAL 1B5 unconjugated

anti-HLA-DR (HLA-DR) antibody
HLA-DR Reactivity: Human, Dog, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC Host: Mouse Monoclonal L243 unconjugated

anti-HLA-DR (HLA-DR) antibody
HLA-DR Reactivity: Human FACS, WB, IP Host: Mouse Monoclonal MEM-12 unconjugated

anti-HLA-DR (HLA-DR) antibody
HLA-DR Reactivity: Human FACS, WB, IHC, IF, ISt, StM Host: Mouse Monoclonal SPM289 unconjugated

anti-HLA-DR (HLA-DR) antibody
HLA-DR Reactivity: Human, Dog, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC, Func Host: Mouse Monoclonal L243 unconjugated

anti-HLA-DR (HLA-DR) antibody
HLA-DR Reactivity: Human FACS, IP Host: Mouse Monoclonal HL-39 unconjugated

anti-HLA-DR (HLA-DR) antibody (PerCP)
HLA-DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-12 PerCP

anti-HLA-DR (HLA-DR) antibody (APC)
HLA-DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-12 APC

anti-HLA-DR (HLA-DR) antibody (PE)
HLA-DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-12 PE

Application Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions

For Research Use only
Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Ivanov, Beers, Walshe, Honeychurch, Alduaij, Cox, Potter, Murray, Chan, Klymenko, Erenpreisa, Glennie, Illidge, Cragg: "Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells." in: The Journal of clinical investigation, Vol. 119, Issue 8, pp. 2143-59, (2009) (PubMed).

De Gassart, Camosseto, Thibodeau, Ceppi, Catalan, Pierre, Gatti: "MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3491-6, (2008) (PubMed).

Swiatek-de Lange, Rist, Stahl, Weith, Lenter: "Comment on "MHC class II expression identifies functionally distinct human regulatory T cells"." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 6, pp. 3625; author reply 3626, (2008) (PubMed).

Muczynski, Ekle, Coder, Anderson: "Normal human kidney HLA-DR-expressing renal microvascular endothelial cells: characterization, isolation, and regulation of MHC class II expression." in: Journal of the American Society of Nephrology : JASN, Vol. 14, Issue 5, pp. 1336-48, (2003) (PubMed).

Bouillon, El Fakhry, Girouard, Khalil, Thibodeau, Mourad: "Lipid raft-dependent and -independent signaling through HLA-DR molecules." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7099-107, (2003) (PubMed).

Kalka-Moll, Tzianabos, Bryant, Niemeyer, Ploegh, Kasper: "Zwitterionic polysaccharides stimulate T cells by MHC class II-dependent interactions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 11, pp. 6149-53, (2002) (PubMed).

Coral, Pucillo, Leonardi, Fonsatti, Altomonte, Maio: "Triggering of HLA-DR antigens differentially modulates tumor necrosis factor alpha release by B cells at distinct stage of maturation." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 8, Issue 5, pp. 581-8, (1997) (PubMed).
Target

HLA-DR

Alternative Name

HLA-DR

Background

HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.

Pathways

Human Leukocyte Antigen (HLA) in Adaptive Immune Response