Insulin Receptor antibody (Middle Region)

Catalog No. ABIN2776774

This Rabbit Polyclonal antibody specifically detects Insulin Receptor in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Horse, Pig, Cow, Guinea Pig and Rabbit.

Quick Overview for Insulin Receptor antibody (Middle Region) (ABIN2776774)

Target: Insulin Receptor (INSR)

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Insulin Receptor Antibody

Binding Specificity: Middle Region

Purpose: INSR Antibody - middle region

Sequence: ENNVVHLMWQ EPKEPNGLIV LYEVSYRRYG DEELHLCVSR KHFALERGCR

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against INSR. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human INSR

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP41374-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: INSR

Background:

Background Information: This receptor binds insulin and has a tyrosine-protein kinase activity. Isoform Short has a higher affinity for insulin. INSR mediates the metabolic functions of insulin. INSR binding to insulin stimulates association of the receptor with downstream media

Gene Name: Insulin receptor

Alternative Symbols: HHF5, CD220

Protein Name: Insulin receptor

Molecular Weight: 154kDa

Gene ID: 3643

NCBI Accession: NP_000199

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity