GRK2 antibody (N-Term)

Catalog No. ABIN2784714

The Rabbit Polyclonal anti-GRK2 antibody has been validated for WB. It is suitable to detect GRK2 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig and Horse.

Quick Overview for GRK2 antibody (N-Term) (ABIN2784714)

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GRK2 Antibody

Binding Specificity: N-Term

Sequence: PEPSIRSVMQ KYLEDRGEVT FEKIFSQKLG YLLFRDFCLN HLEEARPLVE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ADRBK1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ADRBK1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 689 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Alternative Name: ADRBK1

Background: The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.
Alias Symbols: BARK1, BETA-ARK1, FLJ16718, GRK2
Protein Interaction Partner: UBC, SUMO1, NEDD8, Rapgef3, GNAQ, FBXO25, ADRBK2, ACACA, HSP90AB1, HSP90AA1, GIT1, RASGRF1, MDM2, EPHA2, PEBP1, ADRB2, SNCA, GRM1, GNB1, KCNIP3, ARRB1, HDAC6, Nedd4, CSNK2B, PDGFRB, TPM2, GIT2, PDE6G, CCR4, AKAP12, PDCL, NCS1, MAPK3, PRKACA, CCR5, PIK3CG,
Protein Size: 689

Molecular Weight: 79 kDa

Gene ID: 156

NCBI Accession: NM_001619, NP_001610

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling