GTPase NRas antibody (Middle Region)

Catalog No. ABIN2789436

The Rabbit Polyclonal anti-GTPase NRas antibody has been validated for WB. It is suitable to detect GTPase NRas in samples from Human, Rat, Mouse, Dog, Rabbit, Cow, Guinea Pig, Horse and Zebrafish (Danio rerio).

Quick Overview for GTPase NRas antibody (Middle Region) (ABIN2789436)

Target: GTPase NRas (NRAS)

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTPase NRas antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GTPase NRas Antibody

Binding Specificity: Middle Region

Sequence: ADINLYREQI KRVKDSDDVP MVLVGNKCDL PTRTVDTKQA HELAKSYGIP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against NRAS. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 189 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GTPase NRas

Target: GTPase NRas (NRAS)

Alternative Name: NRAS

Background: This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.
Alias Symbols: ALPS4, N-ras, NRAS1, NS6
Protein Interaction Partner: UBC, MDM2, RGL2, VAT1, RNASEH2B, SPATS2, CD2AP, ELAVL1, SRI, RPS20, DNAJB1, EEF1A1, RAP1GDS1, RASGRP2, PLCE1, SMURF2, RASSF5, RACGAP1, SHOC2, SMAD4, ARHGAP4, PIK3CA, RAF1, BCL2, ACVR1, PIK3CG, MLLT4,
Protein Size: 189

Molecular Weight: 21 kDa

Gene ID: 4893

NCBI Accession: NM_002524, NP_002515

UniProt: P01111

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling