Insulin Receptor antibody (INSR) (Middle Region) Primary Antibody
- Insulin Receptor (INSR)
- Middle Region
- Human, Mouse, Rat, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)
- This Insulin Receptor antibody is un-conjugated
- Immunohistochemistry (IHC), Western Blotting (WB)
- SSHCQREEAG GRDGGSSLGF KRSYEEHIPY THMNGGKKNG RILTLPRSNP
- Predicted Reactivity
- Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Rat: 93%, Zebrafish: 86%
- This is a rabbit polyclonal antibody against INSR. It was validated on Western Blot using a cell lysate as a positive control.
- Affinity Purified
- The immunogen is a synthetic peptide directed towards the middle region of human INSR
anti-Insulin Receptor (INSR) (Middle Region) antibody Primary Antibody
INSR Reactivity: Human, Mouse, Rat, Dog, Rabbit, Xenopus laevis, Zebrafish (Danio rerio) IHC, WB Host: Rabbit Polyclonal unconjugated
- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
Antigen size: 1382 AA
- For Research Use only
- Lot specific
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
- Insulin Receptor (INSR)
- Alternative Name
- INSR (INSR Antibody Abstract)
- CD220, HHF5, 4932439J01Rik, D630014A15Rik, IR, IR-A, IR-B, 18402, CG18402, DIHR, DILR, DIR, DIRH, DIRbeta, DInR, DInr, Dir-a, Dir-b, Dmel\\CG18402, INR, INS, Inr, Inr-alpha, Inr-beta, InsR, dINR, dIR, dIRH, dInR, dInr, dInsR, dinr, dir, er10, inr, insulin/insulin-like growth factor receptor, l(3)05545, l(3)93Dj, l(3)er10, lnR, ir-A, CTK-1, ir, INSR, NV14476, cd220, hhf5, insulin receptor, Insulin-like receptor, insulin receptor L homeolog, INSR, Insr, InR, LOC100122567, LOC100451802, insr.L
This receptor binds insulin and has a tyrosine-protein kinase activity. Isoform Short has a higher affinity for insulin. INSR mediates the metabolic functions of insulin. INSR binding to insulin stimulates association of the receptor with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K). INSR can activate PI3K either directly by binding to the p85 regulatory subunit, or indirectly via IRS1. When present in a hybrid receptor with IGF1R, it binds IGF1.A report shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, another report shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: CD220, HHF5
Protein Interaction Partner: KRT31, UBC, ARRB2, MOK, Grb2, Hgf, DOK1, SH2B1, Stat5b, TEAD1, STAT5A, JAK2, JAK1, IRS1, IRF7, PLCG1, GRB10, Socs6, Socs1, SOCS3, SH2B2, HGS, INPPL1, Calmodulin, Mad2l1, KRT27, DOK5, DOK4, SNX6, SNX4, SNX2, ACP1, PTPN2, SYNCRIP, FRS2, PIK3R3, VAV3, ARHGAP
Protein Size: 1382
- Molecular Weight
- 154 kDa
- Gene ID
- NCBI Accession
- NM_000208, NP_000199
- NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity