DFFA antibody (C-Term)

Catalog No. ABIN2842766

Product Details anti-DFFA Antibody

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Binding Specificity: AA 304-331, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DFFA antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This DFFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-331 amino acids from the C-terminal region of human DFFA.

Clone: RB23680

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for DFFA

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Alternative Name: DFFA (DFFA Products)

Synonyms: DFF-45 antibody, DFF1 antibody, ICAD antibody, A330085O09Rik antibody, DFF35 antibody, Dff45 antibody, ICAD-L antibody, ICAD-S antibody, DFFA antibody, zgc:92229 antibody, DNA fragmentation factor subunit alpha antibody, DNA fragmentation factor, alpha subunit antibody, DNA fragmentation factor, 45kDa, alpha polypeptide antibody, DNA fragmentation factor, alpha polypeptide antibody, DFFA antibody, Dffa antibody, dffa antibody

Background: DFFA is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.

Molecular Weight: 36522

Gene ID: 1676

NCBI Accession: NP_004392, NP_998731

UniProt: O00273

Pathways: Apoptosis, Caspase Cascade in Apoptosis