Biotin antibody
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- Target See all Biotin products
- Biotin
- Reactivity
- Please inquire
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Biotin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purification
- Biotin antibody was purified by ion exchange chromatography followed by dialysis.
- Immunogen
- Biotin antibody was raised in goat using biotin conjugated to KLH as the immunogen.
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- Application Notes
- ELISA: 1:100,000-1:500,000, WB: 1:10,000-1:50,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.02 M NaPO4, 0.12 M NaCl, pH 7.2, with 0.01 % NaN3
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- -20 °C
- Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- Target
- Biotin
- Abstract
- Biotin Products
- Target Type
- Chemical
- Background
- Biotin is a water-soluble B-complex vitamin (vitamin B7) that is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme in the metabolism of fatty acids and leucine, and it plays a role in gluconeogenesis.
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