GAD65 antibody (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)) (C-Term)

Catalog No. ABIN2854688

Product Details anti-GAD65 Antibody

Target:

GAD65 (GAD2)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAD65 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Cross-Reactivity: Dog (Canine), Pig (Porcine), Rhesus Monkey

Cross-Reactivity (Details): Dog (92 %), Pig (85 %), Rhesus Monkey (100 %)

Characteristics: Rabbit Polyclonal antibody to GAD65 (glutamate decarboxylase 2 (pancreatic islets and brain, 65 kDa))
GAD65 antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:5000-1:20000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:5000-1:20000*

Comment:

Positive Control: mouse brain , rat brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GAD65

Target:

GAD65 (GAD2)

Alternative Name: GAD65 (GAD2 Antibody Abstract)

Synonyms: GAD65, gad65, F12P19.12, F12P19_12, GLUTAMATE DECARBOXYLASE 2, glutamate decarboxylase 2, zgc:112198, 6330404F12Rik, GAD(65), Gad-2, glutamate decarboxylase 2, glutamate decarboxylase 1, glutamic acid decarboxylase 2, GAD2, PTRG_06148, LOC9324534, Gad2, GAD1, gad2

Background: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Cellular Localization: Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse , presynaptic cell membrane , Golgi apparatus membrane

Molecular Weight: 65 kDa

Gene ID: 2572