PDIA3 antibody (C-Term)

Catalog No. ABIN2854699

Product Details anti-PDIA3 Antibody

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDIA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to ERp57 (protein disulfide isomerase family A, member 3)
ERp57 antibody [C3], C-term

Purification: Affinity purified by Protein A.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ERp57. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , JC , BCL-1 , H1299 , HeLa , HepG2

Validation: Comparison

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PDIA3

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Alternative Name: protein disulfide isomerase family A member 3 (PDIA3 Products)

Synonyms: ER60 antibody, ERp57 antibody, ERp60 antibody, ERp61 antibody, GRP57 antibody, GRP58 antibody, HsT17083 antibody, P58 antibody, PI-PLC antibody, grp-58 antibody, 58kDa antibody, Erp antibody, Grp58 antibody, PDI antibody, PDI-Q2 antibody, PLC[a] antibody, Plca antibody, 1 antibody, 25D3-membrane-associated antibody, sb:cb825 antibody, erp57 antibody, grp58 antibody, pdia3 antibody, protein disulfide isomerase family A member 3 antibody, protein disulfide-isomerase A3 antibody, protein disulfide isomerase associated 3 antibody, protein disulfide isomerase family A, member 3 antibody, protein disulfide isomerase family A member 3 S homeolog antibody, PDIA3 antibody, Tsp_10062 antibody, Pdia3 antibody, pdia3 antibody, pdia3.S antibody

Background: This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase, however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Cellular Localization: Endoplasmic reticulum lumen (By similarity) , Melanosome

Molecular Weight: 57 kDa

Gene ID: 2923

UniProt: P30101

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis