Amphiregulin antibody

Catalog No. ABIN2854897

Product Details anti-Amphiregulin Antibody

Target: Amphiregulin (AREG)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Amphiregulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to Amphiregulin (amphiregulin)
Amphiregulin antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Amphiregulin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: AREG-transfected 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.17 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Amphiregulin antibody (ABIN2854897)

Yaojia Cheng, Lu, Shi, Zhou, Rong, Li, Wang, Liu: "Aberrant expression of the UPF1 RNA surveillance gene disturbs keratinocyte homeostasis by stabilizing AREG." in: International journal of molecular medicine, Vol. 45, Issue 4, pp. 1163-1175, (2020) (PubMed).

Khelwatty, Essapen, Bagwan, Green, Seddon, Modjtahedi: "The impact of co-expression of wild-type EGFR and its ligands determined by immunohistochemistry for response to treatment with cetuximab in patients with metastatic colorectal cancer." in: Oncotarget, Vol. 8, Issue 5, pp. 7666-7677, (2018) (PubMed).

Target Details for Amphiregulin

Target: Amphiregulin (AREG)

Alternative Name: amphiregulin (AREG Products)

Synonyms: AR antibody, CRDGF antibody, SDGF antibody, Sdgf antibody, AREG antibody, AREGB antibody, AREG/SDGF antibody, amphiregulin antibody, AREG antibody, Areg antibody

Background: The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encoded protein is associated with a psoriasis-like skin phenotype.

Cellular Localization: Membrane

Molecular Weight: 28 kDa

Gene ID: 374

UniProt: P15514

Pathways: RTK Signaling, EGFR Signaling Pathway