RANKL antibody (N-Term)

Catalog No. ABIN2856614

Product Details anti-RANKL Antibody

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RANKL antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine)

Cross-Reactivity (Details): Mouse (86 %), Rat (86 %), Bovine (100 %)

Characteristics: Rabbit polyclonal antibody to RANKL (CD254) (tumor necrosis factor (ligand) superfamily, member 11)
RANKL antibody [N1], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human RANKL. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: A431 , HeLa , Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RANKL

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Alternative Name: RANKL (CD254) (TNFSF11 Products)

Synonyms: CD254 antibody, ODF antibody, OPGL antibody, OPTB2 antibody, RANKL antibody, TRANCE antibody, hRANKL2 antibody, sOdf antibody, TNFSF11 antibody, Ly109l antibody, OPG antibody, Trance antibody, cd254 antibody, odf antibody, opgl antibody, optb2 antibody, rankl antibody, sodf antibody, tnfsf11 antibody, trance antibody, TNF superfamily member 11 antibody, tumor necrosis factor (ligand) superfamily, member 11 antibody, TNF receptor superfamily member 11b antibody, TNF superfamily member 11 L homeolog antibody, TNFSF11 antibody, Tnfsf11 antibody, TNFRSF11B antibody, tnfsf11.L antibody

Background: This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

Cellular Localization: Isoform 1: Cell membrane, Single-pass type II membrane protein , Isoform 3: Cell membrane, Single-pass type II membrane protein , Isoform 2: Cytoplasm , Tumor necrosis factor ligand superfamily member 11 , soluble form: Secreted

Molecular Weight: 35 kDa

Gene ID: 8600

Pathways: NF-kappaB Signaling