Nitrotyrosine antibody

Catalog No. ABIN289971

Product Details anti-Nitrotyrosine Antibody

Target: Nitrotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nitrotyrosine antibody is un-conjugated

Application: Please inquire

Purification: The spleen cells from BALB/c mouse, immunized with nitrotyrosin-HSA, were fused tomyeloma P3U1 cells. The screening of the hybridoma cells was performed on ELISA.The cell line was grown on non-serum medium, from which the antibody was purified by Protein G affinity chromatography.

Immunogen: Nitrotyrosine-HSA

Clone: 2H1

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Concentration: 0.25 mg/ml

Buffer: PBS (containing 0.1 % BSA as a stabilizer, 0.1 % Proclin as a bacteriostat)

Preservative: ProClin

Storage: -20 °C

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Abstract: Nitrotyrosine Products

Target Type: Chemical

Background: To elucidate the function of Nitric Oxide (NO) related signal transduction, we developed a new monoclonal antibody to Nitrotyosin (Clone No.2H1). There are two pathways, which isengaged in the signal transduction regarding vascular relaxation on endothelial cell. One isthrough activated guanlyate cyclase that is cGMP dependent and another is cGMPindependent pathway which requires reactive NO derived substance such as,peroxinitrate (ONOO), N2O3,N2O4(NO2).Recently, various functions of reactive NO derived substance has been identified forexample regulating protein phosphorylation and inducing apoptosis. So, it is believed thatreactive NO derived substance is involved in signal transduction among cells.Immunohystochemical study revealed that nitotyrosin residue is produced in some patientsuch as athrosclerosis, Alzheimer