BMI1 antibody (AA 252-264)

Catalog No. ABIN292553

Product Details anti-BMI1 Antibody

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Binding Specificity: AA 252-264

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Brand: IHC-plus™

Specificity: Amino acids 252-264 of human Bmi1 protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human BMI1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:30000), ICC, IF, IHC, IHC-P (2.5 μg/mL), WB (1:500 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI1 / PCGF4 (BMI1 Products)

Synonyms: FLVI2/BMI1 antibody, PCGF4 antibody, RNF51 antibody, AW546694 antibody, Bmi-1 antibody, Pcgf4 antibody, bmi1 antibody, pcgf4 antibody, psc1 antibody, wu:fb17g03 antibody, wu:fd18f06 antibody, BMI-1 antibody, pcgf4b antibody, bmi-1 antibody, bmi1-a antibody, bmi1-b antibody, bmi1b antibody, rnf51 antibody, xbmi-1 antibody, BMI1 proto-oncogene, polycomb ring finger antibody, BMI1 polycomb ring finger oncogene antibody, Bmi1 polycomb ring finger oncogene antibody, bmi1 polycomb ring finger oncogene 1a antibody, bmi1 polycomb ring finger oncogene 1b antibody, BMI1 proto-oncogene, polycomb ring finger L homeolog antibody, BMI1 antibody, LOC100230513 antibody, Bmi1 antibody, bmi1a antibody, bmi1b antibody, bmi1.L antibody

Background: Name/Gene ID: BMI1

Synonyms: BMI1, BMI-1, Flvi-2/bmi-1, FLVI2/BMI1, PCGF4, Polycomb group protein Bmi1, Polycomb complex protein BMI-1, Dna-binding protein bmi-1, Polycomb group ring finger 4, RING finger protein 51, RNF51

Gene ID: 648

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy