IRF3 antibody (AA 206-427)
-
- Target See all IRF3 Antibodies
- IRF3 (Interferon Regulatory Factor 3 (IRF3))
-
Binding Specificity
- AA 206-427
-
Reactivity
- Human
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- This IRF3 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)
- Specificity
- Selected for its ability to recognize human IRF3 in the applications listed below. In direct ELISA and Western Blots, this antibody shows ~60 % cross-reactivity with rmIRF3.
- Purification
- Affinity purified
- Immunogen
- E. coli-derived rhIRF3 (aa206-427).
- Isotype
- IgG
- Top Product
- Discover our top product IRF3 Primary Antibody
-
-
- Application Notes
-
Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), ICC (10 μg/mL), WB (1 μg/mL)
Usage: Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Western Blot: 1 μg/mL (w/ appropriate secondary reagents). Detection limit is ~10 ng/lane (non-reducing) and ~1 ng/lane (reducing). Flow Cytometry: 50 μg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 HeLa cells in a total reaction volume 200 μL. The binding of unlabeled antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as anti-goat IgG (FITC). Immunocytochemistry: 10 μg/mL detects human IRF3 in HeLa cells. Cells were fixed with PBS, 4 % paraformaldehyde for 20 min at RT and blocked with PBS, 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 min at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1 % BSA. ELISA: 0.5-1.0 μg/mL (w/ appropriate secondary reagents), detection limit is ~0.2 ng/well. - Comment
-
Target Species of Antibody: Human
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- 40-50 % glycerol, PBS.
- Concentration
- Lot specific
- Buffer
- Lyophilized.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C,-20 °C
- Storage Comment
- 4°C or -20°C, Avoid freeze-thaw cycles.
-
- Target
- IRF3 (Interferon Regulatory Factor 3 (IRF3))
- Alternative Name
- IRF3 / IRF-3 (IRF3 Products)
- Synonyms
- irf3 antibody, IRF3 antibody, C920001K05Rik antibody, IRF-3 antibody, interferon regulatory factor 3 antibody, interferon regulatory factor 7 antibody, irf3 antibody, IRF3 antibody, irf-3 antibody, Irf3 antibody, IRF7 antibody
- Background
-
Name/Gene ID: IRF3
Synonyms: IRF3, Interferon regulatory factor 3, IRF-3 - Gene ID
- 3661
- UniProt
- Q14653
- Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
-