GTPase NRas antibody (Internal Region)

Catalog No. ABIN302138

The Goat Polyclonal anti-GTPase NRas antibody has been validated for WB and ELISA. It is suitable to detect GTPase NRas in samples from Human.

Quick Overview for GTPase NRas antibody (Internal Region) (ABIN302138)

Target: GTPase NRas (NRAS)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GTPase NRas antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-GTPase NRas Antibody

Binding Specificity: Internal Region

Purpose: NRAS

Sequence: RMKKLNSSDD GTQ

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 21-22 kDa band observed in lysates of cell line MCF7 (calculated MW of 21.2 kDa according to NP_002515.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: A customer obtained weak cytoplasmic staining in MCF7 cells at 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GTPase NRas

Target: GTPase NRas (NRAS)

Alternative Name: NRAS

Background: NRAS , neuroblastoma RAS viral (v-ras) oncogene homolog , N-ras , NRAS1, N-ras protein part 4 , v-ras neuroblastoma RAS viral oncogene homolog

Gene ID: 4893, 18176, 24605

NCBI Accession: NP_002515

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling