ABCC11 antibody (Internal Region)
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- Target See all ABCC11 Antibodies
- ABCC11 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 11 (ABCC11))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This ABCC11 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- MRP8 / ABCC11
- Sequence
- KVVEFDRPEV LRK
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-KVVEFDRPEVLRK, from the internal region (near C Terminus) of the protein sequence according to NP_115972.2, NP_660187.1.
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Breast. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 125 kDa band observed in Human Testis lysates and in preliminary testing of Breast lysate (calculated MW of 150 kDa according to NP_660187.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.
Peptide ELISA: antibody detection limit dilution 1:32000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentrat
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- ABCC11 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 11 (ABCC11))
- Alternative Name
- ABCC11 (ABCC11 Products)
- Synonyms
- EWWD antibody, MRP8 antibody, WW antibody, ATP binding cassette subfamily C member 11 antibody, ABCC11 antibody
- Background
- ABCC11, ATP-binding cassette transporter MRP8, ATP-binding cassette, sub-family C (CFTR/MRP), member 11, EWWD, MRP8, WW, ATP-binding cassette protein C11, ATP-binding cassette, sub-family C, member 11, multi-resistance protein 8
- Gene ID
- 85320
- NCBI Accession
- NP_115972, NP_660187
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