VWF antibody

Catalog No. ABIN3024051

Product Details anti-VWF Antibody

Target: VWF (Von Willebrand Factor (VWF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VWF antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G purified

Immunogen: A recombinant human protein fragment was used as the immunogen for this von Willebrand Factor antibody.

Clone: WFA52-2

Isotype: IgG1 kappa

Application Details

Application Notes: Variations in protocols, secondaries and substrates may require the von Willebrand Factor antibody to be titered for optimal performance.

1. FFPE staining requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.5-1.0 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the von Willebrand Factor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for VWF

Target: VWF (Von Willebrand Factor (VWF))

Alternative Name: Von Willebrand Factor (VWF Products)

Synonyms: VWF antibody, si:ch1073-474e24.1 antibody, F8VWF antibody, VWD antibody, 6820430P06Rik antibody, AI551257 antibody, B130011O06Rik antibody, C630030D09 antibody, von Willebrand factor antibody, Von Willebrand factor antibody, VWF antibody, vwf antibody, Vwf antibody

Background: Von Willebrand Factor (vWF) is a glycoprotein produced by bone marrow cells and endothelial cells lining the inside surface of blood vessels. Its primary function is platelet adhesion, binding to Factor VIII, collagen and platelets, to coagulate blood at the site of wounding. The enzyme VWFCP, or vWF-cleaving protease, facilitates clotting by cutting the protein into subunits, increasing its binding capacity.

Deficiency or dysfunction of the protein increases the tendency of wounds to bleed, or to bleed more.  Over 300 gene mutations have been identified and classified into three types.  Type 1 von Willebrand Factor disease is characterized by reduced amounts in the bloodstream, Type 2 by reduced binding ability and Type 3 by a nonfunctional protein.

Gene ID: 7450