ACTH antibody (AA 1-24)
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- Target See all ACTH Antibodies
- ACTH (Adrenocorticotropic hormone (ACTH))
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Binding Specificity
- AA 1-24
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Reactivity
- Human, Rat, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This ACTH antibody is un-conjugated
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogen
- Amino acids 1-24 (AH26) and an N-terminal peptide from human ACTH (57) were used as the immunogens for this ACTH antibody cocktail.
- Clone
- AH26-57
- Isotype
- IgG1
- Top Product
- Discover our top product ACTH Primary Antibody
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- Application Notes
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The optimal dilution of the ACTH antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the ACTH antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- ACTH (Adrenocorticotropic hormone (ACTH))
- Alternative Name
- ACTH / Synacthen (ACTH Products)
- Synonyms
- ACTH antibody, BE antibody, Beta-LPH antibody, Clip antibody, Gamma-LPH antibody, Npp antibody, Pomc-1 antibody, Pomc1 antibody, alpha-MSH antibody, alphaMSH antibody, beta-MSH antibody, gamma-MSH antibody, CLIP antibody, LPH antibody, MSH antibody, NPP antibody, POC antibody, pro-opiomelanocortin-alpha antibody, proopiomelanocortin antibody, Pomc antibody, POMC antibody
- Target Type
- Hormone
- Background
- ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This cocktail is specific to Synacthen (aa1-24 of ACTH), does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
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