CD74 antibody
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- Target See all CD74 Antibodies
- CD74 (HLA-DR-gamma (CD74))
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Reactivity
- Human, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD74 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogen
- SU-DHL-4 lymphoma cells were used as the immunogen for this CD74 antibody.
- Clone
- LN-2
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD74 Primary Antibody
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- Application Notes
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the CD74 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- CD74 (HLA-DR-gamma (CD74))
- Alternative Name
- CD74 (CD74 Products)
- Synonyms
- DHLAG antibody, HLADG antibody, II antibody, Ia-GAMMA antibody, CLIP antibody, Ii antibody, INVG34 antibody, Iclp-1 antibody, iclp1 antibody, wu:fb11f09 antibody, wu:fb12a01 antibody, wu:fb13d06 antibody, wu:fk94e07 antibody, Iclp-2 antibody, cd74 antibody, iclp2 antibody, zgc:123161 antibody, CD74 molecule antibody, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody, CD74 molecule, major histocompatibility complex, class II invariant chain a antibody, CD74 molecule, major histocompatibility complex, class II invariant chain b antibody, CD74 antibody, Cd74 antibody, cd74a antibody, cd74b antibody
- Background
- CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. CD74 antibody stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. antibody to CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
- Gene ID
- 972
- Pathways
- Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints
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