Melanoma gp100 antibody
-
- Target See all Melanoma gp100 (PMEL) Antibodies
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This Melanoma gp100 antibody is un-conjugated
-
Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- No Cross-Reactivity
- Dog (Canine), Rat (Rattus)
- Purification
- Protein G affinity chromatography
- Immunogen
- The extract of pigmented melanoma metastases from lymph nodes was used as the immunogen for this gp100 antibody.
- Clone
- HMB45
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product PMEL Primary Antibody
-
-
- Application Notes
-
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the gp100 antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western Blot: 1-2 μg/mL, FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
-
- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the gp100 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
- Target
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
- Alternative Name
- Gp100 (PMEL Products)
- Synonyms
- D12S53E antibody, ME20 antibody, ME20-M antibody, ME20M antibody, P1 antibody, P100 antibody, PMEL17 antibody, SI antibody, SIL antibody, SILV antibody, gp100 antibody, D10H12S53E antibody, D12S53Eh antibody, Pmel17 antibody, Si antibody, Silv antibody, gp87 antibody, RPE1 antibody, MMP115 antibody, silverb antibody, silver antibody, cb397 antibody, fdv antibody, pmel17 antibody, sb:cb397 antibody, silva antibody, wu:fc11g11 antibody, wu:fj24g11 antibody, zgc:136622 antibody, premelanosome protein antibody, premelanosome protein b antibody, premelanosome protein a antibody, PMEL antibody, Pmel antibody, pmelb antibody, pmela antibody
- Background
-
Melanocytes produce organelles called melanosomes which produce melanin, the pigment that gives color to skin, hair, eyes, scales and feathers. gp100 was identified in an attempt to clone the gene Tyrosinase, an enzyme required for melanin synthesis. Further testing determined that gp100 is a melanoma-specific protein and is responsible for melanosome maturation, facilitating the transition from amorphous rounded vesicles to fibrillary ellipsoid organelles.
Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. Clone HMB45 gp100 antibody stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. It also stains angiomyolipomas, tumors most commonly associated with the kidney. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. This gp100 antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. - Gene ID
- 6490
-