p53 antibody (N-Term)
Quick Overview for p53 antibody (N-Term) (ABIN3025364)
Target
See all p53 (TP53) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
Clone
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Binding Specificity
- AA 20-25, N-Term
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Purpose
- Anti-p53 Antibody (azide and preservative free)
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No Cross-Reactivity
- Mouse (Murine), Rat (Rattus)
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Purification
- Protein G affinity chromatography
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Immunogen
- Recombinant human wild type p53 protein was used as the immunogen for this anti-p53 antibody. Its epitope maps within the N-terminus (approx. amino acids 20-25).
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Isotype
- IgG2a, kappa
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anti-Tumor Protein P53 (TP53) (pSer392) antibody
TP53 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) antibodyKO Validated TP53 Reactivity: Human WB, IF, IHC (p), IP, ICC, ChIP Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (N-Term) antibodyTP53 Reactivity: Human WB, IF, IHC (p), IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 Reactivity: Human WB, ELISA, IF, IHC (p), IP Host: Mouse Monoclonal 2C3 unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 Reactivity: Human WB, ELISA, IHC (p), PLA, RNAi Host: Mouse Monoclonal 2C11 unconjugated
anti-Tumor Protein P53 (TP53) (pSer20) antibodyTP53 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (acLys319) antibodyTP53 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer15) antibodyTP53 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 301-393) antibodyTP53 Reactivity: Human, Mouse, Rat, Goat WB, ELISA, FACS, IHC (p), ICC, IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 293-322) antibodyTP53 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB7777 unconjugated
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Application Notes
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The optimal dilution of the anti-p53 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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Preservative
- Azide free
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store the anti-p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- p53 (TP53) (Tumor Protein P53 (TP53))
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Alternative Name
- p53
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Background
- P53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis.
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UniProt
- P04637
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Pathways
- p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
Target
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