PCNA antibody

Catalog No. ABIN3025663

The Mouse Monoclonal anti-PCNA antibody (Clone SPM350) (ABIN3025663) specifically detects PCNA in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for PCNA antibody (ABIN3025663)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: SPM350

Product Details anti-PCNA Antibody

Purpose: Anti-PCNA Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Rat PCNA protein was used as the immunogen for this anti-PCNA antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: The optimal dilution of the anti-PCNA antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy