HLA-DR antibody

Catalog No. ABIN3026340

The Mouse Monoclonal anti-HLA-DR antibody is suitable to detect HLA-DR in samples from Human, Cat and Cow. It has been validated for FACS and IF.

Quick Overview for HLA-DR antibody (ABIN3026340)

Target: HLA-DR

Reactivity: Human, Cat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DR antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 169-1B5-2

Product Details anti-HLA-DR Antibody

Characteristics: This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Purification: Protein G affinity chromatography

Immunogen: Human peripheral blood leukocytes were used as the immunogen for the HLA-DR antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal dilution of the HLA-DR antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the HLA-DR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for HLA-DR

Target: HLA-DR

Alternative Name: HLA-DR (MHC II)

Background: This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36  kDa alpha (heavy) chain and a 28  kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response