Cyclin A antibody

Catalog No. ABIN3026638

Product Details anti-Cyclin A Antibody

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivity: Human, Mouse, Cow, Green Monkey, Mink

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin A antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Characteristics: This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.

Purification: Protein G affinity chromatography

Immunogen: Full length bovine protein was used as the immunogen for this CCNA2 antibody.

Clone: E67

Isotype: IgG2a kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin A2 antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Cyclin A2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Alternative Name: Cyclin A2 (CCNA2 Products)

Synonyms: CCN1 antibody, CCNA antibody, AA408589 antibody, Ccn-1 antibody, Ccn1 antibody, Ccna antibody, CycA2 antibody, Cyca antibody, cb671 antibody, chunp6924 antibody, wu:fi36f03 antibody, CYCA antibody, Cyclin-A2 antibody, ccna2 antibody, ccn1 antibody, ccna antibody, ccna1 antibody, Cyclin-A antibody, cyclinA antibody, cyclin A2 antibody, cyclin A2 S homeolog antibody, cyclin A2 L homeolog antibody, CCNA2 antibody, Ccna2 antibody, ccna2 antibody, ccna2.S antibody, ccna2.L antibody

Background: This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.

Gene ID: 890

Pathways: PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA