Topoisomerase II alpha antibody
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- Target See all Topoisomerase II alpha (TOP2A) Antibodies
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Topoisomerase II alpha antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
- Characteristics
- This mAb recognizes a 170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
- Purification
- Protein G affinity chromatography
- Immunogen
- Human partial recombinant protein was used as the immunogen for this TOP2A antibody.
- Clone
- TPM2A-1
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product TOP2A Primary Antibody
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- Application Notes
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissue requires boiling sections in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,Western blot: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL (1)
- Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the TOP2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
- Alternative Name
- TOP2A (TOP2A Products)
- Synonyms
- TOP2 antibody, TP2A antibody, TOP2A antibody, Top-2 antibody, TOP-2 antibody, F12B17.110 antibody, F12B17_110 antibody, thimet metalloendopeptidase 2 antibody, fc08c07 antibody, wu:fc08c07 antibody, DNA topoisomerase II alpha antibody, DNA topoisomerase II, alpha isozyme antibody, topoisomerase (DNA) II alpha antibody, Zincin-like metalloproteases family protein antibody, TOP2A antibody, Bm1_08705 antibody, Top2a antibody, AT5G10540 antibody, top2a antibody
- Background
- This mAb recognizes a 170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
- Gene ID
- 7153
- Pathways
- Cell Division Cycle, Mitotic G1-G1/S Phases
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