HSPB2 antibody (Middle Region)

Catalog No. ABIN3031147

Product Details anti-HSPB2 Antibody

Target: HSPB2 (Heat Shock 27kDa Protein 2 (HSPB2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human HSPB2 (RHPQRLDRHGFVSREFC) was used as the immunogen for this HSPB2 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the HSPB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the HSPB2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for HSPB2

Target: HSPB2 (Heat Shock 27kDa Protein 2 (HSPB2))

Alternative Name: HSPB2 (HSPB2 Products)

Synonyms: zgc:112532 antibody, HSP27 antibody, Hs.78846 antibody, LOH11CR1K antibody, MKBP antibody, 27kDa antibody, 2810021G24Rik antibody, heat shock protein family B (small) member 2 antibody, heat shock protein, alpha-crystallin-related, b2 antibody, heat shock protein 2 antibody, HSPB2 antibody, hspb2 antibody, Hspb2 antibody

Background: Alpha-B-crystallin is a member of the alpha-crystallin/small heat-shock protein (HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al.(1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They called it Heat shock 27 protein 2 or Heat shock protein beta 2 and termed the gene HSPB2. Iwaki et al.(1997) also determined the complete genomic structure of the rat homolog of HSPB2. Southern blot analysis demonstrated that HSPB2 is expressed preferentially in skeletal muscle and heart but not in the lens, while the neighboring CRYABgene is highly expressed in all three tissues. The two related genes are arranged in a head-to-head manner with an intergenic sequence of less than 1 kb, raising a possibility of shared regulatory elements for their expression.

UniProt: Q16082