NRF1 antibody (Middle Region)
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- Target See all NRF1 Antibodies
- NRF1 (Nuclear Respiratory Factor 1 (NRF1))
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Binding Specificity
- Middle Region
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NRF1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Purification
- Antigen affinity
- Immunogen
- An amino acid sequence from the middle region of human Nuclear Respiratory Factor 1 (QHGREDLLYAFEDQQTQ) was used as the immunogen for this NRF1 antibody (100% homologous in human, mouse and rat).
- Isotype
- IgG
- Top Product
- Discover our top product NRF1 Primary Antibody
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- Application Notes
- The stated application concentrations are suggested starting amounts. Titration of the NRF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
- Restrictions
- For Research Use only
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- Buffer
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
- Storage
- -20 °C
- Storage Comment
- After reconstitution, the NRF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- Target
- NRF1 (Nuclear Respiratory Factor 1 (NRF1))
- Alternative Name
- NRF1 (NRF1 Products)
- Synonyms
- ALPHA-PAL antibody, C87038 antibody, D6Ertd415e antibody, SC:BZ3C13.1 antibody, SC:bZ3C13.2 antibody, ube2h antibody, Nfe2l1_retired antibody, IBR/F antibody, NRF1 antibody, alpha-pal antibody, nrf-1 antibody, LOC100220087 antibody, nuclear respiratory factor 1 antibody, nuclear respiratory factor 1 S homeolog antibody, NRF1 antibody, Nrf1 antibody, nrf1 antibody, nrf1.S antibody
- Background
- Nuclear Respiratory Factor 1 is also known as Alpha-Pal. Efiok et al.(1994) identified genes containing NRF1-binding sequences and found that these could be classified either as cellular proliferation genes, or as genes regulating the growth-responsive metabolic pathways of energy transduction, translation, and replication. Virbasius and Scarpulla(1994) noted that the nuclear-encoded mitochondrial transcription factor< a href=http://www.nsjbio.com/search_result.php?search_txt=tfam>TFAMcontains potential binding sites for NRF1, NRF2(GABPA) and SP1 within the promoter region. With use of binding and electrophoretic mobility shift assays, DNase footprinting, and mutation analysis of recombinant proteins, they demonstrated specific and functional binding of< a href=http://www.nsjbio.com/search_result.php?search_txt=nrf1>NRF1and< a href=http://www.nsjbio.com/search_result.php?search_txt=nrf2>NRF2to the TFAM promoter region.
- UniProt
- Q16656
- Pathways
- Regulation of Lipid Metabolism by PPARalpha
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