ORAI1 antibody (C-Term)

Catalog No. ABIN3032099

Product Details anti-ORAI1 Antibody

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORAI1 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human Orai1 (EFARLQDQLDHRGDHPLTPGSHYA) was used as the immunogen for this Orai1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Orai1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Orai1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for ORAI1

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Alternative Name: Orai1 (ORAI1 Products)

Synonyms: CRACM1 antibody, ORAT1 antibody, TMEM142A antibody, RGD1311873 antibody, D730049H07Rik antibody, Tmem142a antibody, orai-1 antibody, im:7146264 antibody, orai1 antibody, tmem142a antibody, zgc:109721 antibody, orai2 antibody, ORAI1 antibody, LOC100218031 antibody, ORAI calcium release-activated calcium modulator 1 antibody, ORAI calcium release-activated calcium modulator 1b antibody, ORAI calcium release-activated calcium modulator 1 S homeolog antibody, ORAI1 antibody, Orai1 antibody, orai1b antibody, orai1.S antibody, orai1 antibody

Background: Orai calcium release-activated calcium modulator 1, also known as CRACM1, and TMEM142A, is a calcium selectiveion channel that in humans is encoded by the ORAI1 gene. Orai1 channels play an important role in the activation of T-lymphocytes. The loss of function/mutation of Orai1 causes Severe combined immunodeficiency (SCID) in humans. The mammalian Orai family has two additional homologs, Orai2 and Orai3. Orai proteins share no homology with any other ion channel family of any other known proteins. They have 4 transmembrane domains and form tetramers. Prakriya et al.(2006) showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of two conserved acidic residues in the transmembrane segments. Glu106-to-asp(E106D) and glu190-to-gln(E190Q) substitutions in transmembrane helices 1 and 3, respectively, diminished calcium ion influx, increased current carried by monovalent cations, and rendered the channel permeable to cesium ion. They showed that Orai1 is a PM protein, and that CRAC channel function is sensitive to mutation of two conserved acidic residues in the transmembrane segments.

UniProt: Q96D31

Pathways: TCR Signaling, BCR Signaling