SMAD3 antibody (SMAD, Mothers Against DPP Homolog 3) (AA 417-425)

Details for Product anti-SMAD3 Antibody No. ABIN303328, Supplier: Log in to see
Antigen
  • madh3
  • madh3a
  • smad3
  • wu:fa99e03
  • HSPC193
  • HsT17436
  • JV15-2
  • LDS1C
  • LDS3
  • MADH3
  • XSmad3
  • XenMLP
  • Xmad3
  • madh3-A
  • Madh3
  • AU022421
  • Smad 3
  • mad3
  • SMAD family member 3
  • SMAD family member 3a
  • SMAD family member 3 L homeolog
  • SMAD3
  • smad3a
  • smad3.L
  • Smad3
Alternatives
anti-Human SMAD3 antibody for Western Blotting
Epitope
AA 417-425, pSer423, pSer425
71
60
52
49
38
36
22
22
19
18
18
16
12
11
11
10
10
9
8
8
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5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Frog, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
616
333
317
56
47
41
38
32
23
19
18
4
3
3
2
2
1
1
1
1
Host
Rabbit
559
64
15
Clonality
Polyclonal
Conjugate
This SMAD3 antibody is un-conjugated
24
22
20
12
12
11
8
8
7
7
7
5
5
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
487
273
263
134
111
67
57
36
31
19
18
8
7
2
2
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Synthetic peptide corresponding to amino acids 417-425 of Human SMAD3 protein.
Specificity This antibody recognises SMAD3 pSer423/425.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Chicken, Frog, Mouse, Pig, Rat and Zebrafish.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography.
Plasmids, Primers & others Plasmids, Primers & others SMAD3 products on genomics-online (e.g. as negative or positive controls)
Alternative Name SMAD3 (SMAD3 Antibody Abstract)
Background Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-b-mediated response.Synonyms: JV15-2, MAD homolog 3, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, SMAD family member 3, SMAD-3
Gene ID 4088
NCBI Accession NP_001138574
UniProt P84022
Pathways Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
Application Notes ELISA (1/25000-1/100000). Western Blot (1/2000-1/20000). Immunofluorescence. Immunohistochemistry on Paraffin Sections (2.5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (ABIN303328) anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody
Product cited in: Sun, Zhou, Gui, Shimokado, Nakanishi, Oikawa, Sato, Muragaki: "Elevated serum 1,25(OH)2-vitamin D3 level attenuates renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction in kl/kl mice." in: Scientific reports, Vol. 4, pp. 6563, 2014 (PubMed).

Carracedo, Lu, Popova, Jonsson, Eckes, Gullberg: "The fibroblast integrin alpha11beta1 is induced in a mechanosensitive manner involving activin A and regulates myofibroblast differentiation." in: The Journal of biological chemistry, Vol. 285, Issue 14, pp. 10434-45, 2010 (PubMed).

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